Identification of peroxiredoxin II and its related molecules as potential biomarkers of dermal mesenchymal stem cell homing using network analysis

Abstract In this study, we performed RNA sequencing of Prx II +/+ and ?/? dermal mesenchymal stem cells (DMSCs) to identify differentially expressed genes (DEGs). To explore the role in DMSCs, Gene Ontology analysis DEGs. The results showed that DEGs were mainly involved biological processes cell migration, intercellular adhesion, coordination regulation homing. Through construction protein–protein interaction network, four hub Cd274 , Ccl5 Il1b Stat1 adhesion homing screened. Quantitative reverse transcription PCR down regulated DMSCs. miRwalk Starbase databases further used screen upstream molecules miRNA lncRNA regulating gene. was found be via Tctn2/miR-351/Stat1/Il1b axis. Thus, demonstrated is a key molecule ability Our provide theoretical foundation for improving DMSCs by targeting II.